Figure 2 | Scientific Reports

Figure 2

From: A novel method of rapid detection for heavy metal copper ion via a specific copper chelator bathocuproinedisulfonic acid disodium salt

Figure 2

Optimization of BCS-Cu+ binding assay. (A) Schematic depiction of BCS binding with cuprous ion. (B) Absorption spectra of Cu+, BCS and Cu+-BCS. (C) The effect of ascorbate on BCS assay (50 mM Tris–HCl pH 7.4, 200 µM BCS, 25 μM CuSO4). (D) The effect of BCS on BCS assay (50 mM Tris–HCl pH 7.4, 25 μM CuSO4, 1 mM ascorbate). (E) The effect of CuSO4 on BCS assay (50 mM Tris–HCl pH 7.4, 200 µM BCS, 1 mM ascorbate). (F) The effect of pH on BCS assay (200 µM BCS, 25 μM CuSO4, 1 mM ascorbate). All assays were conducted at least 6 times. Average ± S.D. is presented. Asterisk * indicates significant difference (* p < 0.05, **p < 0.01).

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