Figure 3

Identification of BCS binding specificity by spectrophotometer. Absorbance spectrum (350–650 nm) measurements under optimized reaction conditions (50 mM Tris–HCl pH 7.4, 200 μM BCS, 1 mM ascorbate) of BCS binding with Cu⁺ (A), Cd²⁺ (B), Cr³⁺ (C), Hg²⁺ (D), Ni²⁺ (E) and Pb²⁺ (F) by spectrophotometer.