Figure 1

AP induction leads to activation of the MYD88/IRAK3 signalling pathway in macrophages. AP was induced by hourly injection of Caerulein (Caer) over a period of 8 h. (A) Flow cytometry of isolated leukocytes from pancreas showed a significant increase of TLR4/MD2⁺ CD11b⁺ cells (con n = 4, 8 h Caer n = 7). (B) qRT PCR showed a significantly increased expression of the Irak3 gene (n = 3). (C) BMDMs co-incubated for 6 h with freshly prepared acini showed also a significantly increased expression of the Irak3 gene (con n = 5 + acini n = 7). (D) Co-incubation of BMDMs from wild type and IRAK3 deficient mice with freshly prepared acini for 6 h showed a significantly increased expression of pro-inflammatory genes, such as Tnfa (n = 3), Il6 (n = 6), Nos2 (n = 3) and Il12b (n = 6). (E) Secretion of the pro-inflammatory cytokines IL-6 and TNFα in supernatant was also increased in IRAK3 deficient BMDMs (n = 4). Significant differences were tested using student’s t-test, all error bars indicate SEM. Significance level p < 0.05 is marked by an asterisk.