Figure 3
From: Mechanosensitive traction force generation is regulated by the neutrophil activation state
RMS tractions and traction maxima of neutrophils from healthy donors after stimulation by PMA. Mean (and SEM) of (a) root-mean-square tractions (\(T_{RMS}\)) and (b) traction maxima (\(T_{Max}\)) of neutrophils from healthy donors seeded on 1.5 kPa polyacrylamide gels (n = 70). Cells were allowed to attach and settle and then imaged before stimulation (“Unstim.”). Cells were then treated with 20nM Phorbol Myristate Acetate (“PMA”) and imaged 30 min post-addition. Cells were maintained at 37 °C throughout the duration of the experiment. Displacements were computed in Matlab by T-PT, and displacement heatmaps were generated for a representative cell at 30 min (c); tractions were computed using the finite element method in Abaqus. Scale bars are 10 μm, and the white contours represents the boundary of the cell edge. ***p < 0.001, ****p < 0.0001 (paired Student’s t-test).
