Figure 7
From: Mechanosensitive traction force generation is regulated by the neutrophil activation state
Mechanosensitivity to substrate stiffness is differentially dependent on the activating stimulant. Mean (and SEM) of RMS tractions (\(T_{RMS}\)) and traction maxima (\(T_{Max}\)) of neutrophils from healthy donors seeded on polyacrylamide gels with a Young’s modulus of either 1.5 kPa or 10 kPa. Cells were allowed to attach and settle, and then imaged before stimulation (“Unstim.”). Cells were then treated with either: (a–d) 100 ng/ml LPS (1.5 kPa, n = 34; 10 kPa, n = 30); (e–h) a cytokine cocktail of 100 μg/ml IFN-\(\gamma\) 100 ng/ml GM-CSF (1.5 kPa, n = 63; 10 kPa, n = 52); or (i–l) a combined cocktail of LPS, IFN-\(\gamma\), and GM-CSF (1.5 kPa, n = 34; 10 kP,a n = 52). Cells imaged 5 min post-addition and 30 min post-addition. Tractions were computed using the finite element method in Abaqus. Analyzed using paired Student’s t-test comparing each 5 min timepoint to the unstimulated timepoint and comparing each 30 min timepoint to the unstimulated timepoint, *p < 0.05.
