Figure 3

Comparison of SARS-CoV-2 growth in IEC#17 and other IECs. (A) The growth of SARS-CoV-2 was compared in intestinal epithelial cells differentiated from induced pluripotent stem cells (iPSCs) (IEC#17; black) and primary IECs derived from jejunum (red), ileum (green) and colon (blue). Cells were infected at an MOI of 0.1. The supernatants were collected at the indicated time points. SARS-CoV-2 growth was measured by determining the TCID₅₀ on VeroE6/TMPRSS2 cells. Each value is representative of at least three independent experiments and is shown as the mean ± SD from three wells of supernatants of each culture group. The significant differences were determined using two-way ANOVA. (B) SARS-CoV-2 titers in IECs differentiated from various iPSCs. The IECs were infected with SARS-CoV-2 at an MOI of 0.1. The supernatants were collected at 48 hpi. SARS-CoV-2 growth was measured by determining the TCID₅₀ on VeroE6/TMPRSS2 cells. Each value is representative of at least three independent experiments and is shown as the mean ± SD from three wells of supernatants of each culture group. *0.01 < P < 0.05; **0.005 < P < 0.01; ***P < 0.005.