Figure 6
From: Numerical and experimental investigation of multi-species bacterial co-aggregation
Co-aggregates and biofilms. a–c Low magnification phase-contrast micrographs of cell suspensions with a magnified subsection showing bacterial cells. From left: Streptococcus gordonii strain \(SPS\_017\), S. gordonii strain \(SPS\_017\) mixed with Eikenella corrodens strain \(SPS\_010\), and E. corrodens strain \(SPS\_010\). Both strains originate from the same severe case of peri-implant disease. After 24 h of incubation large co-aggregates were observed in the mixture but not in monospecies suspensions. Image fragments at high magnification are included in the top left corners. d–e Confocal micrographs of 8-h biofilms representing monoculture or co-culture biofilms are shown in the same order as in a–c. Bigger aggregates were observed in mixed-biofilms compared to monospecies biofilms. Scale bars, \(4\,\upmu \hbox {m}\) (a–c, small bar), \(10\,\upmu \hbox {m}\) (a-c, large bar), and \(15\,\upmu \hbox {m}\) (d–e).
