Table 1 Overview of flow cytometry antibodies used.
From: Identification of circulating monocytes as producers of tuberculosis disease biomarker C1q
Marker | Fluorophore | Clone | Concentration | Vendor |
|---|---|---|---|---|
Live/dead | Fixable Blue | – | 1:1000 | Invitrogen |
CD3 | BV510 | SK7 | 1:50 | Biolegend |
CD4 | PerCP/Cy5.5 | SK3 | 1:50 | BD |
CD8 | Af700 | RPA-T8 | 1:50 | BD |
CD14 | PE | M5E2 | 1:50 | BD |
CD16 | PE/Cy5 | 3G8 | 1:100 | Biolegend |
CD19 | BV570 | HIB19 | 1:50 | Biolegend |
CD56 | PE/Cf594 | NCAM16.2 | 1:50 | BD |
C1q | FITC | polyclonal | 10 µL/sample | Dako |
