Figure 1 | Scientific Reports

Figure 1

From: von Willebrand factor-binding protein (vWbp)-activated factor XIII and transglutaminase 2 (TG2) promote cross-linking between FnBPA from Staphylococcus aureus and fibrinogen

Figure 1

Expression of vWbp during bacterial growth and protein detection in sera from patients with infective endocarditis. (A) At the indicated times, the bacterial culture supernatants were collected, filtered and then immobilized onto microtiter wells. The presence of vWbp in the wells was revealed using anti-vWbp IgG followed by secondary HRP-conjugated IgG (right axis). Bacterial growth curve is also reported (left axis). (B) To test the immunological reactivity against vWbp, microtiter wells coated with vWbp were probed with IgG isolated from sera o human patients affected by staphylococcal endocarditis (P). IgG from healthy donors were used as controls (C2, C7). Bound antibody was detected by the addition of secondary HRP-conjugated IgG to the wells. Statistically significant differences between patients and controls are indicated (*P < 0.05; **P < 0.01; ***P < 0.001). Data are expressed as means ± S.D. of tests performed in triplicate.

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