Figure 4 | Scientific Reports

Figure 4

From: von Willebrand factor-binding protein (vWbp)-activated factor XIII and transglutaminase 2 (TG2) promote cross-linking between FnBPA from Staphylococcus aureus and fibrinogen

Figure 4

vWbp-activated FXIII elicits cross-links formation between region A of FnBPA and α-chain of Fbg. Panel (A) shows cross-linking of recombinant FnBPA N1N2N3 domain to Fbg by action of vWbp-activated FXIII. After indicated time intervals mixtures were separated by SDS-PAGE under reducing conditions and transferred to a PVDF membrane. The membrane was probed with anti-FnBPA IgG followed by secondary HRP-conjugated IgG. (B) Cross-links formation between FnBPA N1N2N3 domain and Fbg by vWbp-activated FXIII was analyzed by incubating the membrane with anti-α chain Fbg IgG followed by secondary HRP-conjugated IgG. Arrows show the positions of the α chain or α chain/FnBPA N1N2N3 complexes. (C-E) Cross-linking formation of the recombinant A domain of FnBPB (C), ClfA (D) or ClfB (E) with Fbg by vWbp-activated FXIII were analyzed incubating the PVD membranes with mouse FnBPB, ClfA or ClfB IgG, respectively, followed by secondary HRP-conjugated IgG. The figures are representative of three independent experiments. Molecular masses of standard proteins are indicated on the left side of each panel. Incubation times (0–120 min) on the top of each panel are also specified. Original blots are presented in Supplementary Figure 3.

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