Figure 5 | Scientific Reports

Figure 5

From: von Willebrand factor-binding protein (vWbp)-activated factor XIII and transglutaminase 2 (TG2) promote cross-linking between FnBPA from Staphylococcus aureus and fibrinogen

Figure 5

N1 subdomain of region A of FnBPA is involved in cross-link formation with Fbg. Mixtures containing FnBPA N2N3 (A) or FnBPA N1 (B) proteins and Fbg were incubated in the presence of vWbp-activated FXIII for the indicated times (top of each panel) and then separated by SDS-PAGE under reducing conditions and transferred to a PVDF membrane. Membranes were probed with FnBPA IgG followed by secondary HRP-conjugated IgG. Arrows show the positions of FnBPA N2N3 domain (A) or FnBPA N1 subdomain (B). The figures are representative of three independent experiments. Molecular masses of standard proteins are indicated on the left side of each panel. Original blots are presented in Supplementary Figure 4.

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