Figure 6 | Scientific Reports

Figure 6

From: von Willebrand factor-binding protein (vWbp)-activated factor XIII and transglutaminase 2 (TG2) promote cross-linking between FnBPA from Staphylococcus aureus and fibrinogen

Figure 6

TG2 promotes formation of cross-links between N1 domain of FnBPA and Fbg. Products of cross-linking reactions between FnBPA N1N2N3 (A), N1 (B) and N2N3 (C) proteins and Fbg generated by action of TG2 were separated by SDS-PAGE under reducing conditions and transferred to a PVDF membrane. Membranes were probed with anti-FnBPA IgG followed by secondary HRP-conjugated IgG. Arrows show the positions of unreactive FnBPA proteins. The figures are representative of three independent experiments. Molecular masses of standard proteins are indicated on the left side of each panel. Incubation times (0–120) of the mixtures are indicated on the top of each panel. Original blots are presented in Supplementary Figure 5.

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