Table 1 List of bacterial strains used in this study.

From: von Willebrand factor-binding protein (vWbp)-activated factor XIII and transglutaminase 2 (TG2) promote cross-linking between FnBPA from Staphylococcus aureus and fibrinogen

Bacterial strain

Relevant properties

Reference

S. aureus

 LAC wt

Community-associated MRSA of USA300 lineage

53

 LAC spa

Strain derivative of LAC* deficient in protein A; constructed by transduction of spa::Kanr by phage 85 into strain LAC*

54

 LAC srtA

Constructed by the transduction of srtA::Ermr from Newman srtA using bacteriophage 85

11

 LAC fnbAfnbB (pFnBA)

fnbA fnbB double mutant transformed with plasmid-expressing FnBPA

55

 LAC fnbAfnbB (pFnBB)

fnbA fnbB double mutant transformed with plasmid-expressing FnBPB

55

E. coli

 BL21 (DE3)

E. coli cloning host

Invitrogen

  1. Kanr, kanamycin resistance; Ermr, erythromycin resistance.