Table 1 List of bacterial strains used in this study.
Bacterial strain | Relevant properties | Reference |
---|---|---|
S. aureus | ||
LAC wt | Community-associated MRSA of USA300 lineage | |
LAC spa | Strain derivative of LAC* deficient in protein A; constructed by transduction of spa::Kanr by phage 85 into strain LAC* | |
LAC srtA | Constructed by the transduction of srtA::Ermr from Newman srtA using bacteriophage 85 | |
LAC fnbAfnbB (pFnBA) | fnbA fnbB double mutant transformed with plasmid-expressing FnBPA | |
LAC fnbAfnbB (pFnBB) | fnbA fnbB double mutant transformed with plasmid-expressing FnBPB | |
E. coli | ||
BL21 (DE3) | E. coli cloning host | Invitrogen |