Table 1 CCD and ANN of process factors with coded and actual levels, experimental and predicted values of AuNPs biosynthesis using the cell-free supernatant of Streptomyces flavolimosus.

From: Artificial neural network approach for prediction of AuNPs biosynthesis by Streptomyces flavolimosus, characterization, antitumor potency in-vitro and in-vivo against Ehrlich ascites carcinoma

Std	Run	Type	X₁	X₂	X₃	X₄	AuNPs biosynthesis (µg/mL)
							Actual	CCD		ANN
							Actual	Predicted	Residuals	Predicted	Residuals
2	1	Fact	1	− 1	− 1	− 1	356.17	328.06	28.11	360.58	4.41
22	2	Axial	0	0	2	0	866.29	845.4	20.88	870.36	4.07
15	3	Fact	− 1	1	1	1	604.77	609.52	− 4.75	605.58	0.81
27	4	Center	0	0	0	0	559.57	575.71	− 16.14	576.26	16.69
3	5	Fact	− 1	1	− 1	− 1	272.23	247.95	24.28	268.04	− 4.19
26	6	Center	0	0	0	0	588.63	575.71	12.91	576.26	− 12.37
13	7	Fact	− 1	− 1	1	1	709.33	715.06	− 5.73	709.66	0.33
16	8	Fact	1	1	1	1	717.77	732.56	− 14.78	716.99	− 0.78
17	9	Axial	− 2	0	0	0	540.2	528.51	11.69	540.75	0.55
8	10	Fact	1	1	1	− 1	556.34	551.85	4.5	556.66	0.32
6	11	Fact	1	− 1	1	− 1	756.36	734.86	21.49	755.98	− 0.38
1	12	Fact	− 1	− 1	− 1	− 1	382	371.1	10.9	381.04	− 0.96
9	13	Fact	− 1	− 1	− 1	1	398.72	379.85	18.87	397.25	− 1.47
12	14	Fact	1	1	− 1	1	385.23	355.71	29.51	384.85	− 0.38
4	15	Fact	1	1	− 1	− 1	278.68	276.84	1.85	256.93	− 21.75
30	16	Center	0	0	0	0	556.34	575.71	− 19.37	576.26	19.92
24	17	Axial	0	0	0	2	378.77	373.48	5.29	380.08	1.31
18	18	Axial	2	0	0	0	577.35	608.52	− 31.17	583.70	6.35
21	19	Axial	0	0	− 2	0	194.83	235.19	− 40.37	195.48	0.65
10	20	Fact	1	− 1	− 1	1	269.59	257.52	12.06	269.06	− 0.53
25	21	Center	0	0	0	0	588.63	575.71	12.91	576.26	− 12.37
5	22	Fact	− 1	− 1	1	− 1	598.31	604.46	− 6.15	598.54	0.23
11	23	Fact	− 1	1	− 1	1	380.74	406.11	− 25.37	381.75	1.01
19	24	Axial	0	− 2	0	0	583.02	612.6	− 29.58	585.24	2.22
14	25	Fact	1	− 1	1	1	765.25	766.16	− 0.9172	761.20	− 4.05
28	26	Center	0	0	0	0	572.48	575.71	− 3.23	576.26	3.78
7	27	Fact	− 1	1	1	− 1	333.57	349.51	− 15.94	335.31	1.74
20	28	Axial	0	2	0	0	465.94	455.85	10.1	467.10	1.16
29	29	Center	0	0	0	0	588.63	575.71	12.91	576.26	− 12.37
23	30	Axial	0	0	0	− 2	159.23	184.01	− 24.78	160.23	1.00

Variable	Code	− 2	− 1	0	1	2
Temperature (°C)	X₁	25	30	35	40	45
Incubation time (days)	X₂	2	3	4	5	6
HAuCl₄ conc. (µg/mL)	X₃	200	400	600	800	1000
Initial pH level	X₄	4	5	6	7	8

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Table 1 CCD and ANN of process factors with coded and actual levels, experimental and predicted values of AuNPs biosynthesis using the cell-free supernatant of Streptomyces flavolimosus.

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