Figure 1

Generation of humanized hACE2 NIKO mice. (A) Percentages of human CD45⁺ lymphocytes in blood, spleen and lungs of mice at 12-weeks post-transplantation of CD34⁺ hematopoietic stem cells. Data representative of 2 independent in vivo experiments. Data are represented as mean ± SEM. (B) and (C) Validation of human ACE2 (hACE2) expression in human lung cancer cell line A549 by RT-PCR. Data representative of 2 independent in vitro experiments. (B) Validation of human ACE2 (hACE2) expression in human lung cancer cell line A549 by immunohistochemistry. Scale bar represents 100 µm and images are representative from two independent in vitro experiments (C). (D) Confirmation of hACE2 expression in humanized hACE2 NIKO mouse lungs by immunohistochemistry. Scale bar represents 200 µm and images are representative from 2 independent in vivo experiments.