Figure 4

Humanized mice transduced with hACE2 AdV exhibits inflammatory responses when infected with SARS-CoV-2. Peak responses of (A) Viral antigen (spike protein), human immune cell infiltration, human CD45⁺ lymphocytes and CD3⁺ T cells are present in humanized hACE2 NIKO mice infected with SARS-CoV-2 or SARS-CoV-2-∆382 at day 7. Scale bar represents 100 µm and images are representative from 2 independent in vivo experiments. (B) Staining intensity in humanized hACE2 NIKO mice infected with ∆382 as compared to control and WT virus as observed in histological stains of Viral antigen (spike protein), human cell infiltration, human CD45⁺ lymphocytes and CD3⁺ T cells.