Table 1 AMOVA and genetic analyses of Amphistegina lobifera genotypes with two or more populations (Ia, IIa and IIc).
Dataset | Alignment | Population | Oceanicbasin | Sequences | AMOVA | Nucleotide diversity | Number haplotypes | Number segregating sites | Tajima's D statistic | Tajima (p) | ||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Variation | Df | Variation (%) | Phi st | Phi st (p) permuted | ||||||||||
SSU | Genotype Ia | Mauritius | West-Ind | 11 | 0.122 | < 0.001 | 0.0078 | 158 | 252 | − 2.92 | 0.003 | |||
Zanzibar | West-Ind | 23 | Among oceanic basins | 4 | − 8.6* | |||||||||
Falasarna | Crete | 6 | Among populations | 5 | 20.8 | |||||||||
Nahsholim | East-Med | 3 | Within populations | 429 | 87.8 | |||||||||
Shikmona | East-Med | 72 | Total | 438 | ||||||||||
Avola | Sicily | 61 | ||||||||||||
Capo Passero | Sicily | 76 | ||||||||||||
Plemmirio | Sicily | 70 | ||||||||||||
Marzamemi | Sicily | 33 | ||||||||||||
Eilat | Red Sea | 84 | ||||||||||||
Total | 439 | |||||||||||||
Genotype IIa | Kimbe-Bay | South-Pacific | 19 | Among oceanic basins | 1 | 9.9 | 0.133 | < 0.001 | 0.031 | 68 | 149 | − 1.249 | 0.211 | |
Lizard-Island | South-Pacific | 19 | Among populations | 2 | 3.4 | |||||||||
Palau | Pacific Ocean | 41 | Within populations | 107 | 86.7 | |||||||||
Ponhpei | Pacific Ocean | 32 | Total | 110 | ||||||||||
Total | 111 | |||||||||||||
Genotype IIc | Ningaloo-reef | Indian Ocean | 16 | Among oceanic basins | 1 | 40.1 | 0.433 | < 0.001 | 0.025 | 33 | 115 | − 1.582 | 0.113 | |
Indonesia | Pacific Ocean | 11 | Among populations | 1 | 3.2 | |||||||||
Japan | Pacific Ocean | 24 | Within populations | 48 | 56.7 | |||||||||
Total | 51 | Total | 50 | |||||||||||
SSU + ITS | Genotype Ia | Shikmona | East-Med | 72 | Among oceanic basins | 2 | − 12.5* | 0.135 | < 0.001 | 0.019 | 125 | 612 | − 2.36 | 0.018 |
Avola | Sicily | 61 | Among populations | 2 | 26.0 | |||||||||
Capo Passero | Sicily | 76 | Within populations | 356 | 86.5 | |||||||||
Plemmirio | Sicily | 70 | Total | 360 | ||||||||||
Eilat | Red Sea | 82 | ||||||||||||
Total | 361 |