Figure 6

Rules for interpretation of real-time PCR results of suspected C. includens, C. chalcites, or C. eriosoma samples based on Cq values. 1Inconclusive result is likely due to insufficient DNA quantity or poor DNA quality. 2Inconclusive result is likely due to contamination, artifacts, or unsampled genotype. 3CO1 barcoding should be carried out to delineate between C. chalcites and C. eriosoma when needed.