Table 1 Primers and probes used in this study.
Name | Description | Sequence | Tm1 | Source |
|---|---|---|---|---|
Lep_rDNA_F | rDNA forward primer | 5′-CGATACCGCGAATGGCTC-3′ | 58.1 | This study |
Lep_rDNA_R | rDNA reverse primer | 5′-GTTCAGGCATAATCCCGC-3′ | 55.9 | This study |
Includens_F | C. includens real-time forward primer | 5′-CGCATATGACGGTGCTCC-3′ | 58.4 | This study |
Includens_P | C. includens real-time probe | 5′-/56-FAM/ATTGGGTGC/ZEN/TTCGCGTTCGC/3IABkFQ/-3′ | 62.5 | This study |
Includens_R | C. includens real-time reverse primer | 5′-CCACAAGCCGTTCGACAA-3′ | 56.3 | This study |
Chalcites_F | C. chalcites/C. eriosoma real-time forward primer | 5′-CGCTGATCGTTCGTCTCG-3′ | 58.4 | This study |
Chalcites_P | C. chalcites/C. eriosoma real-time probe | 5′-/5HEX/CGCGTTCGT/ZEN/TAATCCCGTCTCGT/3IABkFQ/-3′ | 66.6 | This study |
Chalcites_R | C. chalcites/C. eriosoma real-time reverse primer | 5′-CGTCTACGAACACCGCGT-3′ | 58.4 | This study |
Chryso_ITS1_F | Chrysodeixis ITS1 forward primer | 5′-GTTGCTGGGAAGTTGACCA-3′ | 57.5 | This study |
Chryso_ITS1_R | Chrysodeixis ITS1 reverse primer | 5′-GCCCTCAGACAGGAGTGG-3′ | 60.8 | This study |
RT-18S-F2 | 18S real-time control forward primer | 5′-ACCGCCCTAGTTCTAACCGTAAA-3′ | 62.9 | |
RT-18S-P2 | 18S real-time control probe | 5′-/Quasar670/TGTCATCTAGCGATCCGCCGA/BHQ/-3′ | 63.5 | |
RT-18S-R2 | 18S real-time control reverse primer | 5′-CCGCCGAGCCATTGTAGTAA-3′ | 63.2 | |
LepF1 | CO1 barcoding forward primer | 5′-ATTCAACCAATCATAAAGATATTGG-3′ | 57.6 | |
LepR1 | CO1 barcoding reverse primer | 5′-TAAACTTCTGGATGTCCAAAAAATCA-3′ | 60.1 |
