Figure 2
Effect of S. ruber and S. iranica SM (100 µg/mL) on cancer stem cell properties of breast cancer cell lines after 48h treatment. (a) Sphere formation. 1. Sphere formation in S. ruber. 2. Sphere formation in S. iranica. 3. Percentage of sphere formation in S. ruber. 4. Percentage of sphere formation in S. iranica. 5. Size of spheres in S. ruber. 6. Size of spheres in S. iranica. (b) Colony formation. 1. Colony formation in S. ruber. 2. Colony formation in S. iranica. 3. Percentage of colony formation in S. ruber. 4. Size of colonies in S. ruber. 5. Percentage of colony formation in S. iranica. 6. Size of colonies in S. iranica. 7. The shape of colonies in S. ruber. 8. The shape of colonies in S. iranica. 9. Percentage of holoclones in colonies in S. ruber. 10. Percentage of meroclones in colonies in S. ruber. 11. Percentage of paraclones in colonies in S. ruber. 12. Percentage of holoclones in colonies in S. iranica. 13. Percentage of meroclones in colonies in S. iranica. 14. Percentage of paraclones in colonies in S. iranica. (c) Migration and invasion. 1. Migration test in S. ruber. 2. Invasion test in S. ruber. 3. Migration test in S. iranica. 4. Invasion test in S. iranica. 5. Migration number of Breast cancer cell lines in S. ruber. 6. Invasion number of Breast cancer cell lines in S. ruber. 7. Invasion index of Breast cancer cell lines in S. ruber. 8. Migration number of Breast cancer cell lines in S. iranica. 9. Invasion number of Breast cancer cell lines in S. iranica. 10. Invasion index of Breast cancer cell lines in S. iranica. n = 4, **p < 0.01, ***p < 0.001.
