Figure 3
Effect of S. iranica SM on apoptosis and cell cycle of breast cancer cell lines and Tumorigenicity of the SM in breast cancer mice model. (a) Effects on apoptosis. 1. Apoptosis test of Salinivenus iranica SM on breast cancer cells. The number of cells in early (downright quadrate) and late (upright quadrate) apoptosis increased in treated cells in comparison with controls. 2. The percentage of early apoptosis. 3. The percentage of late apoptosis. 4. Quantitative real-time PCR analysis of CASP3 in breast cancer cell lines. 5. Quantitative real-time PCR for SOX2 (a pluripotency gene) in breast cancer cell lines. (b) Effects on cell cycle. 1. The cell cycle of Salinivenus iranica SM on breast cancer cells. 2. The number of cells in the S phase of the cell cycle after 48 h treatment with 100 µg/mL of Salinivenus iranica. 3. The number of cells in the G1 phase was up-regulated in MDA-MB-468 (p < 0.5) and MCF-7 (p < 0.001) significantly but was not changed on MDA-MB-231. 4. The number of cells in the G2 phase was up-regulated in MDA-MB-468 significantly (p < 0.05) but was not changed in MDA-MB-231 and MCF-7 cells. (c) Tumorigenicity in breast cancer mice model. 1. Viability of mouse breast cancer cell line (4T1) in presence of 10, 100, and 200 µg/mL of Salinivenus iranica SM. 2. Breast cancer mice models and their breast cell line tumors in control and treated (SM injected) groups. 3. Mean tumor volume of 4T1 cell line. 4. Quantitative real-time PCR for ki67 (a marker of proliferation), sox2 (a pluripotency gene) and cdh1 (a migration-related gene) in 4T1 tumors. n = 4, *p < 0.05, **p < 0.01, ***p < 0.001.
