Figure 5
Anticancer activity of purified phenol amine molecule. (a) Finding the IC50 dose of phenol amine molecule on breast cancer cell lines (MDA-MB-231, MDA-MB-468 and, MCF-7) by MTT assay. The viability of cancer cells in 48 h reduced to 50% after treatment with a 1 µg/mL dose of phenol amine molecule. (b) Quantitative real-time PCR for CASP3 (a marker of apoptosis), SOX2 (a pluripotency gene) in breast cancer cell lines. Overexpression and downregulation of CASP3 and SOX2 were detected, respectively. n = 4, **p < 0.01, ***p < 0.001.
