Figure 5
Determination of ALT in the liver model. (a-d) ALT release in vascular and hepatic supernatants of TVX-treated (a-b) and LVX-treated (c-d) models at various conditions: control (Ctrl, 0.1% DMSO), 10 µM staurosporine (Stauro), TVX and LVX at a concentration of 1 µM, 10 µM or 20 µM for up to 72 h in the liver model. (e–f) Comparison of ALT release in vascular supernatants 48 h (e) and 72 h (f) after treatment with TVX or LVX (20 µM). The quantified ALT concentration (pg/mL) was plotted as mean ± SD of 3 independent experiments (n = 3). *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001 (Two-way ANOVA with Dunnett’s multiple comparison test (a-d), Two-tailed t test (e–f)).
