Figure 2
From: Propionic acid induces alterations in mitochondrial morphology and dynamics in SH-SY5Y cells
Propionic acid (PPA) leads to dynamic mitochondrial remodeling to maintain network integrity. SH-SY5Y cells were cultured, treated with 3 and 5 mM PPA for 24 h and stained using TMRE and Hoechst 33342 before by MEL analysis. (a) Representative images captured during time-lapse microscopy depict colored and binarized maximum intensity projections at time frame 2 (t2) for each condition. Selected regions indicated in each binary image were enhanced and shown in 3D at three different time frames (t1-t3) to illustrate the dynamic changes over time; fusion events are highlighted in green; fission events are shown in red. (b) Average number of dynamic events at each condition. (c) Average number of mitochondrial structures per cell. (d) Average volume per mitochondrial structure per cell (µm3). Data shown represent n = 15 cells per treatment group. Error bars shown represent mean ± SEM, scale bar = 10 µm, * p < 0.05.
