Figure 3 | Scientific Reports

Figure 3

From: The acquisition order of leukemic drug resistance mutations is directed by the selective fitness associated with each resistance mechanism

Figure 3

BCR::ABL1 transcript expression and copy number rise and fall over dasatinib exposure. (a) BCR::ABL1 gene transcription and (b) DNA copy number in K562 DasR intermediates was determined by qPCR. Transcription data is presented as ΔCt relative to GUSB, and DNA quantity is presented as a percentage of GUSB gene amplification. Both data are reported as mean –/ + SD. Significance was determined by Student’s t-test with Welch’s correction. (c) Sanger sequencing of the BCR::ABL1 kinase domain was performed in K562 DasR intermediates. Highlighted region indicates the nucleotide location p.T315, with the p.T315I mutation first detectable in the K562 25 nM Das intermediate, and expanding with increasing TKI exposure.

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