Figure 3
Para activity limiting Plasmodium infection is microbiota dependent. (A) 16srDNA relative fold change was measured by qPCR in samples collected from antibiotic treated and non-treated backgrounds. In the non-treated background, Para silencing leads to increased level of the total abundance of An. coluzzii bacteria as compared to the dsGFP control group (dotted line), whereas in antibiotic treated background this effect is abolished. The ratio of the normalized 16srDNA in dsPara/dsGFP was calculated using triplicates. *: Statistical p-value related to the deltaCt distribution between dsPara and dsGFP on 3 independent biological replicates are mentioned in the graph. (B) Infection prevalence between dsPara and dsGFP in antibiotic (ATB)-treated background was measured at d-8post infection and showed no statistical difference between the two conditions dsPara and dsGFP. Combined p-value (Fisher method) from the 3 independent biological replicates was obtained for the infection prevalence. n = Total number of dissected mosquitoes. Values obtained in every biological replicate are depicted by dots and dotted lines.
