Figure 4
The cellular model of pulmonary fibrosis induced by TGF-β1. (a) Pulmonary fibroblasts were isolated from lung tissue using an adherent culture method, and subsequently, TGF-β1 was employed to induce their transformation into pulmonary fibrotic cells. (b) Pulmonary fibroblasts were purified from lung tissue and cultured for cell passage. Images were taken at × 40 magnification. Scale bar: 100 μm. (c) Following treatment with TGF-β1 (10 mg/L), lung fibroblasts underwent induction and acquired a fibrotic phenotype. Images of the induced cells were taken at × 40 magnification. Scale bar: 100 μm. (d) qRT-PCR analysis of gene expression supports the formation of a cellular model of pulmonary fibrosis following induction by TGF-β1. Expression of TGF-β1, α-SMA, Col I and E-cadherin levels (n = 3 per group). Data are representative of 5 independent experiments, mean ± SD. n = 5 per experimental group. *P < 0.05; **P < 0.01, ***P < 0.001, 1-way ANOVA followed by Fisher’s LSD post hoc analysis.
