Figure 3
From: Endothelial CD34 expression and regulation of immune cell response in-vitro
Kinetics of CD34 expression over time. CD34 sorted HUVECs were reseeded at a density of 2 × 104 cells/cm2 and CD34 expression was monitored over time (A). To evaluate the effect of confluence and cell proliferation on CD34 expression, magnetically sorted CD34high HUVECs (B) were seeded at a high density of 5 × 104 cells/cm2 or 1 × 104. CD34 expression was evaluated by flow cytometry. Endothelial markers were studied by immunostaining of the umbilical cord vein. In physiological conditions, all endothelial cells expressed CD31 (C) and CD34 (D). In the umbilical cord, very few cell were Ki67 positive and proliferating. These cells were at the periphery of the endothelium (E).
