Figure 4
From: Endothelial CD34 expression and regulation of immune cell response in-vitro
PCA mapping of the transcriptomic results, showing two distinct classes of cells based on the expression of CD34 (A). The results are represented in a volcano plot (adjusted p value vs. log2(fold change)) (B). Hierarchical clustering of the genes represented as heatmap (C). Figures B and C represent the genes which fulfill the following criteria: adjusted p value < 0.1 and Fold Change (FC) > = 4 or < = 0.25. Several genes (CD34, IL-33, IL-1RL1 and ANGPT2) identified by the transcriptomic results were validated by RT-qPCR in ECFCs (D) and HUVECs (E), after a magnetic cell sorting.
