Figure 6

CD4⁺ lymphocyte polarization, induced by CD34 sorted HUVECs. CD34^high and CD34^low HUVECs were seeded at a high confluence and after adhesion, they were co-cultured with PBMCs. Percentage of different CD4⁺ T lymphocytes, including Th17 (IL-17⁺ cells in CD4⁺T cells) (A), Th1 (IFNγ⁺ cells in CD4⁺T cells) (B), and Tregs (% CD127^lowCD45RA⁻FoxP3^high in CD4⁺T cells) (C) was evaluated at day 4 (C) and day 5 (A,B). PBMC alone (PBMC only) were used as a control. The results are shown as before and after representation and each dot correspond to an experiment (*p < 0.05, **p < 0.01: two-tailed Wilcoxon test).