Figure 2
From: Subtractive panning for the isolation of monoclonal PEPITEM peptide antibody by phage display
Expression of PEPI-AC and AC. (a) Western blot of the protein extracted from different fraction. M: Opti-XL protein marker (abm), 1: PEPI-AC periplasmic crude, 2: PEPI-AC cytoplasmic crude, 3: PEPI-AC pellet, 4: AC periplasmic crude, 5: AC cytoplasmic crude, 6: AC pellet. The proteins were probed with Streptavidin HRP (1:5000). Majority of the proteins were remained in the pellet. Purification of (b) PEPI-AC and (c) AC were analysed on 12% SDS-PAGE. M: Opti-protein XL marker (abm), FT: flow-through, W: wash, E1-7: elution fraction 1–7. The original blot and gel images are presented in Supplementary FigS. S2–S4.
