Figure 6
From: C3H/HeNSlc mouse with low phospholipid transfer protein expression showed dyslipidemia
The sequence of endogenous retrovirus inserted into the intron 12 of the Pltp gene altered mRNA construction in the C3H-S mice. (A) The intron 12 of the Pltp gene was amplified from C3H-C and C3H-S mouse genomic DNA using the forward (F) and reverse (R) primers (arrowhead). F and R primers were located on exon 12 and intron 12, respectively. The predicted PCR product size was 1373 bp (dashed arrow). (B) Insertion of the ERV into intron 12 of the Pltp gene. gag-pol putative gag-pol protein gene, IAP intracisternal A-type particle, LTR Long terminal repeat, TSD Target site duplication. (C) The cloning of Pltp cDNA 3′ ends. 3′RACE was performed using primers on exon 9 (1st PCR) and exon 12 (2nd PCR). (D) The predicted size of 2nd PCR product was S3 (C3H-C type). (E) The cDNA construction and predicted amino acid (aa) size of S1, S2, and S3. *stop codon. Original gels in (A) and (D) are presented in Supplementary Fig S5.
