Table 3 Multilocus characterization of Giardia duodenalis isolates based on bg, gdh, and tpi sequences.
Farm | Age | Cattle ID | SSU rRNA (GenBank accession no.) | gdh (GenBank accession no.) | tpi (GenBank accession no.) | bg (GenBank accession no.) | MLG type | Mix |
|---|---|---|---|---|---|---|---|---|
Tumd left banner 1 | Preweaned calves | T1–2 | OP189375 (E) | OP189521 (E1) | OP189568 (E1) | |||
T1–4 | OP189376 (E) | |||||||
T1–15 | OP189377 (E) | |||||||
Postweaned calves | T1–22 | OP189378 (A) | ||||||
T1–23 | OP189379 (E) | OP189606 (E2) | ||||||
T1–26 | OP189380 (E) | OP189550 (A1) | OP189569 (E1) | A + E | ||||
T1–28 | OP189381 (E) | |||||||
T1–30 | OP189382 (A) | OP189640 (A1) | OP189570 (E1) | A + E | ||||
T1–32 | OP189383 (E) | |||||||
T1–36 | OP189384 (A) | |||||||
T1–38 | OP189385 (E) | OP189641 (E1) | ||||||
T1–39 | OP189476 (A) | OP189551 (E1) | A + E | |||||
T1–44 | OP189386 (E) | |||||||
T1–46 | OP189387 (E) | OP189611 (E23) | ||||||
T1–47 | OP189388 (E) | OP189649 (E2) | OP189571 (E4) | |||||
T1–48 | OP189389 (E) | OP189605 (E4) | ||||||
T1–49 | OP189390 (E) | |||||||
T1–51 | OP189391 (E) | OP189642 (E12) | OP189631 (E1) | |||||
T1–52 | OP189476 (E) | |||||||
T1–53 | OP189392 (E) | OP189670 (E1) | ||||||
T1–54 | OP189393 (A) | OP189522 (A1) | OP189572 (A1) | |||||
T1–56 | OP189394 (E) | OP189607 (E4) | ||||||
T1–57 | OP189395 (E) | |||||||
T1–58 | OP189396 (E) | OP189552 (E1) | ||||||
T1–60 | OP189397 (E) | OP189650 (E2) | OP189553 (E23) | OP189573 (E1) | MLG E12 | |||
Young cattle | T1–105 | OP189480 (E) | ||||||
T1–110 | OP189410 (E) | OP189643 (E10) | OP189554 (E1) | OP189576 (E8) | MLG E22 | |||
T1–111 | OP189481 (E) | |||||||
T1–112 | OP189411 (E) | OP189633 (E3) | ||||||
T1–113 | OP189482 (E) | OP189651 (E6) | OP189614 (E4) | |||||
T1–120 | OP189483 (E) | |||||||
T1–122 | OP189484 (E) | |||||||
T1–125 | OP189485 (E) | |||||||
T1–130 | OP189412 (E) | OP189555 (E1) | OP189615 (E1) | |||||
T1–131 | OP189413 (E) | OP189556 (E2) | ||||||
T1–133 | OP189414 (E) | |||||||
T1–139 | OP189486 (E) | OP189566 (E5) | OP189634 (E1) | |||||
T1–140 | OP189487 (E) | |||||||
Adult cattle | T1–63 | OP189515 (E) | OP189632 (E1) | |||||
T1–64 | OP189398 (E) | |||||||
T1–68 | OP189399 (E) | |||||||
T1–69 | OP189400 (E) | OP189523 (E2) | ||||||
T1–70 | OP189401 (E) | |||||||
T1–76 | OP189402 (E) | |||||||
T1–78 | OP189478 (E) | |||||||
T1–79 | OP189403 (E) | OP189612 (E1) | ||||||
T1–80 | OP189404 (E) | |||||||
T1–82 | OP189405 (E) | |||||||
T1–84 | OP189479 (E) | |||||||
T1–90 | OP189406 (E) | OP189574 (E11) | ||||||
T1–96 | OP189407 (E) | |||||||
T1–97 | OP189516 (E) | |||||||
T1–98 | OP189408 (E) | OP189575 (E12) | ||||||
T1–99 | OP189409 (E) | OP189524 (E2) | OP189608 (E19) | |||||
Total | – | – | E (49), A (5) | E (7), A (1) | E (10), A (2) | E (20), A (1) | ||
Tumd left banner 2 | Preweaned calves | T2–1 | OP189417 (E) | OP189664 (E1) | OP189525 (E5) | OP189577 (E9) | MLG E1 | |
T2–2 | OP189418 (E) | |||||||
T2–6 | OP189419 (E) | OP189609 (E3) | ||||||
T2–12 | OP189489 (E) | |||||||
T2–21 | OP189420 (E) | |||||||
T2–22 | OP189421 (E) | OP189665 (E1) | OP189526 (E19) | OP189578 (E1) | MLG E2 | |||
T2–25 | OP189422 (E) | |||||||
T2–29 | OP189423 (E) | OP189666 (E2) | OP189527 (E19) | OP189579 (E5) | MLG E13 | |||
Postweaned calves | T2–31 | OP189424 (E) | OP189644 (E5) | OP189561 (E20) | OP189610 (E1) | MLG E21 | ||
T2–38 | OP189425 (E) | OP189580 (E1) | ||||||
T2–42 | OP189426 (E) | OP189667 (E3) | OP189562 (E2) | OP189581 (E4) | MLG E19 | |||
T2–43 | OP189427 (E) | OP189668 (E2) | OP189563 (E1) | OP189603 (E2) | MLG E14 | |||
T2–45 | OP189428 (E) | OP189529 (E21) | ||||||
T2–46 | OP189429 (E) | OP189672 (E5) | OP189528 (E2) | |||||
T2–49 | OP189430 (E) | OP189582 (E9) | ||||||
Young cattle | T2–54 | OP189431 (E) | ||||||
T2–57 | OP189432 (E) | |||||||
T2–63 | OP189433 (E) | |||||||
T2–65 | OP189490 (E) | |||||||
T2–68 | OP189434 (E) | OP189669 (E1) | OP189530 (E16) | OP189583 (E4) | MLG E8 | |||
Adult cattle | T2–73 | OP189491 (E) | OP189613 (E15) | |||||
T2–85 | OP189492 (E) | OP189531 (E3) | OP189604 (E5) | |||||
T2–97 | OP189435 (A) | |||||||
T2–99 | OP189488 (E) | OP189532 (E13) | OP189584 (E18) | |||||
T2–103 | OP189415 (E) | OP189674 (E8) | OP189533 (E22) | OP189616 (E20) | MLG E23 | |||
T2–105 | OP189416 (E) | OP189638 (E1) | OP189635 (E1) | |||||
Total | – | – | E (25), A (1) | E (10) | E (12) | E (15) | ||
Horinger county | Preweaned calves | 0 | ||||||
Postweaned calves | He-101 | OP189461 (A) | ||||||
He-103 | OP189462 (E) | OP189648 (E11) | OP189567 (E10) | OP189596 (E3) | MLG E25 | |||
He-104 | OP189463 (E) | OP189547 (E15) | OP189597 (E1) | |||||
He-107 | OP189464 (E) | |||||||
He-108 | OP189465 (E) | OP189598 (E1) | ||||||
He-112 | OP189466 (E) | OP189671 (E4) | OP189548 (E2) | OP189599 (E6) | MLG E20 | |||
He-117 | OP189467 (E) | |||||||
He-118 | OP189468 (E) | |||||||
He-120 | OP189469 (E) | OP189675 (E1) | OP189549 (E1) | OP189630 (E10) | MLG E9 | |||
Young cattle | He-62 | OP189455 (E) | ||||||
He-63 | OP189456 (E) | OP189595 (E3) | ||||||
He-65 | OP189512 (E) | OP189647 (E7) | OP189545 (E18) | OP189626 (E17) | MLG E24 | |||
He-66 | OP189457 (E) | |||||||
He-78 | OP189458 (E) | |||||||
He-89 | OP189459 (E) | OP189546 (E11) | ||||||
He-92 | OP189513 (E) | OP189629 (E2) | ||||||
He-93 | OP189460 (A) | |||||||
He-96 | OP189514 (E) | |||||||
Adult cattle | He-26 | OP189448 (E) | OP189593 (E1) | |||||
He-29 | OP189449 (E) | |||||||
He-32 | OP189450 (E) | |||||||
He-35 | OP189451 (E) | OP189627 (E22) | ||||||
He-43 | OP189452 (E) | OP189625 (E6) | ||||||
He-54 | OP189453 (E) | |||||||
He-55 | OP189511 (E) | OP189594 (E1) | ||||||
He-56 | OP189454 (A) | OP189628 (E1) | A + E | |||||
Total | – | – | E (23), A (3) | E (4) | E (6) | E (13) | ||
Togtoh county | Preweaned calves | 0 | ||||||
Postweaned calves | Tuo-31 | OP189436 (E) | OP189652 (E1) | OP189534 (E17) | OP189585 (E7) | MLG E3 | ||
Tuo-32 | OP189437 (E) | OP189653 (E1) | OP189535 (E4) | OP189586 (E7) | MLG E6 | |||
Tuo-33 | OP189438 (E) | OP189654 (E2) | OP189536 (E1) | OP189587 (E13) | MLG E16 | |||
Tuo-34 | OP189439 (E) | OP189655 (E1) | OP189537 (E5) | OP189588 (E14) | MLG E10 | |||
Tuo-36 | OP189440 (E) | OP189656 (E1) | OP189538 (E12) | OP189589 (E5) | MLG E4 | |||
Tuo-39 | OP189493 (E) | OP189657 (E2) | OP189539 (E6) | OP189590 (E3) | MLG E17 | |||
Tuo-40 | OP189494 (E) | OP189639 (E1) | OP189564 (E1) | OP189636 (E3) | MLG E11 | |||
Tuo-41 | OP189441 (E) | OP189658 (E1) | OP189540 (E20) | OP189591 (E1) | MLG E5 | |||
Tuo-42 | OP189442 (E) | OP189659 (E1) | OP189541 (E4) | OP189592 (E7) | MLG E6 | |||
Tuo-44 | OP189443 (E) | OP189660 (E1) | OP189565 (E9) | |||||
Tuo-45 | OP189444 (E) | OP189661 (E1) | OP189557 (E7) | OP189617 (E24) | MLG E7 | |||
Young cattle | Tuo-71 | OP189499 (A) | OP189662 (E2) | OP189558 (A1) | A + E | |||
Tuo-73 | OP189500 (A) | OP189559 (E8) | OP189623 (A1) | A + E | ||||
Tuo-74 | OP189501 (E) | |||||||
Tuo-75 | OP189447 (E) | OP189645 (E2) | OP189542 (E14) | OP189600 (E2) | MLG E15 | |||
Tuo-76 | OP189502 (E) | |||||||
Tuo-84 | OP189503 (E) | OP189663 (E2) | OP189543 (E1) | OP189601 (E1) | MLG E18 | |||
Tuo-85 | OP189518 (E) | OP189619 (E4) | ||||||
Tuo-87 | OP189519 (E) | OP189544 (E1) | OP189602 (E1) | |||||
Tuo-88 | OP189504 (E) | |||||||
Tuo-89 | OP189505 (E) | OP189637 (E3) | ||||||
Tuo-90 | OP189520 (E) | OP189560 (E20) | ||||||
Adult cattle | Tuo-51 | OP189445 (E) | OP189618 (E21) | |||||
Tuo-53 | OP189495 (E) | |||||||
Tuo-54 | OP189496 (E) | |||||||
Tuo-57 | OP189497 (E) | |||||||
Tuo-60 | OP189498 (A) | OP189622 (E16) | A + E | |||||
Tuo-68 | OP189517 (E) | OP189673 (E9) | ||||||
Tuo-70 | OP189446 (E) | |||||||
Tuo-92 | OP189506 (E) | OP189646 (E1) | OP189620 (E1) | |||||
Tuo-93 | OP189507 (E) | |||||||
Tuo-94 | OP189508 (E) | |||||||
Tuo-95 | OP189509 (E) | |||||||
Total | – | – | E (30), A (3) | E (16) | E (16), A (1) | E (18), A (1) | ||
Dalad banner | Preweaned calves | 0 | ||||||
Postweaned calves | 0 | |||||||
Young cattle | Da-6 | OP189470 (A) | ||||||
Da-7 | OP189471 (E) | OP189621 (E4) | ||||||
Da-8 | OP189472 (E) | OP189624 (E4) | ||||||
Adult cattle | Da-11 | OP189473 (E) | ||||||
Da-15 | OP189474 (E) | |||||||
Da-17 | OP189475 (E) | |||||||
Total | – | – | E (5), A (1) | 0 | 0 | E (2) | ||
Hanggin rear banner | Preweaned calves | 0 | ||||||
Postweaned calves | 0 | |||||||
Young cattle | 0 | |||||||
Adult cattle | Ba-4 | OP189510 (E) | ||||||
Total | – | – | E (1) | 0 | 0 | 0 | ||
Total | – | E (133), A (13) | E (37), A (1) | E (44), A (3) | E (68), A (2) | |||
