Figure 4
The effects of sapanisertib (10−7 M); PHA-793887 (10−6 M); ciclopirox (10−6 M); sulfasalazine (10−6 M); cytarabine (10−5 M); 17β-estradiol (10−5 M); and cisplatin (10−5 M) on caspase 3/7 activity and cellular metabolism in EC cells. The Ishikawa, HEC-1B, and KLE cells were treated with drugs for 48 h as described in methods. (A), (F), (K)—Caspase 3/7 activity. (B), (G), (L)—Extracellular glucose concentration. (C), (H), (M)—extracellular lactate concentration. (D), (I), (N)—extracellular glutamine concentration. (E), (J), (O)—extracellular glutamate concentration. (A)–(E)—Ishikawa cells. (F)–(J)—HEC-1B cells. (K)–(O)—KLE cells. Data are shown as the mean ± SEM of three independent experiments run in triplicates. Levels of significance difference (Mann–Whitney U-test) when compared with control: *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.
