Figure 1
Diagram of in vivo experimental protocol. Arrowheads and blue circles indicate measuring the body weights and the amounts of experimental tooth movement of rats, respectively. Red circles indicate the euthanization of rats (a). Schematic of experimental tooth movement in rat. A 0.012″ nickel-titanium wire (red line) was bent into a U-shape (red line) and placed the between the maxillary first molars on both sides. Arrows indicate the direction of tooth movement. M1, first molar; M2, second molar; M3, third molar (b). A representative image of plaster model of maxilla of rat for measurement of the amount of experimental tooth movement. The distance between the tips of mesial palatal cusps (Black dots) of right and left maxillary first molars was measured (Black line). Both sides of maxillary first molars were surrounded by dotted line. M1, first molar. Scale Bar, 1 mm (c). Representative image of a horizontal section of the disto-buccal root of a maxillary first molar. The distance of white line was measured as the PDL width of the compression side of tooth movement and black line as that of the tension side. The area surrounded by white lines (150 × 200 µm) is the region of interest (ROI) for measurement on the compression side of tooth movement. The area surrounded by black lines (150 × 200 µm) is the ROI of the tension side. The arrow indicates the direction of tooth movement, and the dotted line is parallel to the direction of tooth movement through the center of the pulp cavity. P, pulp cavity; D, dentin; PDL, periodontal ligament; AB, alveolar bone. Scale bar, 200 µm (d).
