Figure 2 | Scientific Reports

Figure 2

From: Identification and evolution of nsLTPs in the root nodule nitrogen fixation clade and molecular response of Frankia to AgLTP24

Figure 2

Phylogenetic tree representing nsLTPs evolution in the RNF clade. A graphical representation of the unrooted maximum-likelihood phylogenetic tree of nsLTPs from RNF plants was constructed with IQ-TREE. Typed nsLTPs are represented by colored circles. The unannotated ones are those that do not correspond to any type. The triangles represent the nsLTPs that are up-regulated in functional nodules, of M. truncatula (MtnsLTPs), P. andersonii (PaLTPs), A. glutinosa (AgLTPs) and D. glauca (DgLTPs). For A. glutinosa, AgLTPs up-regulated in nodules compared to non-infected roots are represented by qRT-PCR data (this study). For the tree other plant, up-regulated MtnsLTPs, PaLTPs and DgLTPs in functional nodules were retrieved from databases (see “Materials and methods”). Names of nsLTPs already described in the literature as involved in RNF symbiosis are specified next to the gene name. The scale bar represents the number of substitutions per site (under the selected evolutionary model). The schematic representation of conserved protein motifs predicted using the MEME suite is shown outside the tree.

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