Figure 2

Neuronal orientation relative to the lemniscal fibers. (a) Examples of two electroporated INLL neurons. Scale bar equals 100 µm. (ai and aii) Left: Reconstructions from neurons shown in (a). Wedges used to extract dendritic length in a given orientation are indicated by gray lines. Right: Summed dendritic length of a given wedge presented as polar graph. Axis values are in µm. (b) Average dendritic length of all reconstructed INLL neurons (transversal: n = 25, black and sagittal: n = 17, gray) as a function of orientation. Axis values are in µm. (c) Polar plots of single cells at their position within a schematized INLL in the transversal plane. Sizes of polar plots are normalized to the maximal extension. (d) Overview of the position of reconstructed cells in the tranversal INLL. All labelled and reconstructed cells were added to the schematized INLL nucleus area (gray surrounding) and scaled to a similar proportion. Green compartments are axonal structures.