Figure 5
Distribution of synaptic input on dendrites of INLL neurons. (a) Single filled, reconstructed neuron (red) after re-slicing and labelling for VGlutT1/2 (green) and GlyT2 (blue). White squares indicate the position of the magnified insets. In the insets contact sites are marked with a white asterisk. Scale bars equal 50 µm and 5 µm for insets. (b) Digitalized neuron and the shells for the Sholl analysis for the neurons shown in (a). (c) Contact site number and dendritic surface within the Sholl radii. (d) Inter-contact distances for VGluT1/2 (VGluT) and GlyT2 labelled contact sites for the neuron shown in (a). Inter-contact distance is measured as the Euclidian distance between two contact sites on one dendrite. Inter-contact site distances across different dendrites were not taken into account. Box-plot shows median and quartiles. (e) Average inter-contact site distances. Each color corresponds to data from one neuron. (f) Number of VGluT1/2 and GlyT2 labelled contact sites along the sampled neurons (n = 8). Color code as in (e). (g) Top: Normalized contact site and dendritic surface distributions as a function of Sholl radius for the example neuron. Bottom: Difference between the normalized contact site (con.) and dendritic surface distributions as a function of Sholl radius. (h) Sum of the squared difference of contact site (con.) and dendritic surface distributions as shown in (g) bottom. Color code as in (e).
