Figure 2
Glipr1, Clec12a, and Phlda3 with aging. (a) Gene expressions of Glipr1, Clec12a, and Phlda3 were analyzed by RT-qPCR in the 3-, 6-, 12-, 24-, and 32–34-month-old mouse livers (n = 5, all ages). Three technical replicates were conducted for each sample. Bar plots show relative mRNA expression levels in 6-, 12-, 24-, and 32–34-month-old mouse livers compared to 3-month-old mouse livers. β-actin was used as the endogenous control gene. (b) Correlation coefficients calculated from the expression levels of Glipr1, Clec12a, Phlda3, Cdkn2a, and β-actin in transcriptome data of 7-month-old and 27-month-old rat hepatocytes. The hyphen indicates a pair of genes whose correlation coefficient cannot be calculated by no expression data. (c) Representative images stained for Glipr1, Clec12a, Phlda3, and Alb mRNA in 6- and 27-month-old primary mouse hepatocytes using fluorescence in situ hybridization (FISH). Green dots indicate Glipr1, Clec12a, and Phlda3 mRNA signals. The Alb mRNA is shown as red dots. Nuclei were stained by DAPI (blue). Scale bar = 50 μm. (d) Glipr1 protein levels were analyzed using western blot analysis in 3-, 6-, 12-, 24-, and 32–34-month-old mouse livers (n = 5, all ages). Upper panels are representative chemiluminescence and stain-free images. Uncropped images are shown in Supplementary Fig. S7a. The right panel shows quantitative data normalized by whole protein levels detected by stain-free. Values are presented as the means ± SEM. The statistical analysis was performed using one-way ANOVA followed by Dunnett’s post hoc test. *p < 0.05, ***p < 0.001, and ****p < 0.0001. a.u., arbitrary units.
