Table 1 Yields of REGN10987-Fab and FabH3 from 20 mL cultures in shake flasks.

From: Design of an alternate antibody fragment format that can be produced in the cytoplasm of Escherichia coli

Culture media

Strain

REGN10987 Fab (mg/L)

REGN10987 FabH3 (mg/L)

Rich autoinduction media

BL21 (DE3)

64 ± 4

143 ± 4

MG1655

64 ± 3

128 ± 1

Chemically defined minimal autoinduction media

BL21 (DE3)

–

46 ± 3

  1. The protein yields are expressed in mg/L of culture media. See Supplementary Figs. S5 and S6 for uncropped SDS-PAGE gel images used for protein quantification.
Back to article page