Figure 4

Volcano plot and heatmap. (A) Heatmap of top 50 metabolites of all groups; (B) Heatmap of top 50 metabolites of STR group, MMI group, and SMI group; (C) volcano plot and heatmap of top 50 metabolites in CON vs. STR group; (D) Volcano plot and heatmap of top 50 metabolites in MMI vs. SMI group. In volcano plot, the red dots represent up-regulated, the blue dots represent down-regulated, and the grey dots represent no significant difference (namely, metabolites that are detected but did not meet the filtering parameters for screening). R language “limma” package (http://www.bioconductor.org/packages/release/bioc/html/limma.html) was applied to identify diferentially expressed genes.