Figure 5
From: Asarinin attenuates bleomycin-induced pulmonary fibrosis by activating PPARγ
GW9662 and Pparg silencing inhibited the effect of asarinin on α-SMA and type I collagen expression in myofibroblast transition. Western blotting (WB) and quantitative real-time PCR (Q-PCR) verified the effect of Pparg silencing (A,B). WB detected the protein expression levels of α-SMA and type I collagen after Pparg silencing or GW9662 co-incubation (E–H). Q-PCR was performed to detect the gene expression levels of Acta2 with Col1a1 after Pparg silencing or GW9662 co-incubation (C,D,I,J). Data are expressed as mean ± standard deviation, and all experiments were repeated independently at least 3 times, sample size (n) = 3 for each group, *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.
