Figure 2 | Scientific Reports

Figure 2

From: In-depth proteome analysis of brain tissue from Ewsr1 knockout mouse by multiplexed isobaric tandem mass tag labeling

Figure 2

Quantitative proteomics analysis of Ewsr1 KO and WT mice. (A) Principle-component analysis (PCA) plot. (B) Coefficient variance (CV) plot from raw data of each individual MS analysis. Volcano plots for differential protein expression. Differential expression selected by peptide abundance comparison followed with (C) width adjustment normalization and (D) total peptide amount normalization. (E) Differential expression selected by MSstatsTMT. The x-axis of volcano plot indicates \({\mathrm{log}}_{2}(\mathrm{fold change})\) and two dashed vertical lines mark the 1.2-fold change cutoff and indicate statistical significance; − log10(p-value) and the dashed horizontal line equals to p = 0.05. (F) Common DEPs from measuring peptide abundance based on width adjustment and total adjustment normalization and monitoring each PSMs by MSstatsTMT.

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