Figure 3
Hierarchical clustering of common DEPs in three different normalization methods. (A) Heatmap representation of the 118 common DEPs of Ewsr1 WT and KO mice with colorimetric scheme. Each column represents mice sample and each row represents protein. Hierarchical clustering and heatmap generation were conducted using Perseus software. Hierarchical clustering was perform using the normalized protein intensities calculated from MSstatsTMT. Two major clusters were detected based on protein expression and overall expression aspects of those. The first cluster contains the proteins which were significantly down regulated with Ewsr1 KO and second cluster contains significantly down regulated proteins by Ewsr1 KO. DAVID analysis results of cluster 1 (B) and cluster 2 (C). The p-value was presented with the bar chart and protein counts were indicated with line charts. The dashed line in (B,C) represents p < 0.05.
