Figure 2

The effects of Tet-inducible artificial microRNAs on cell apoptosis in bladder cancer cells. The relative activity of caspase-3 was calculated in T24/5637 cells treated with “amiRNA β-catenin” (A,B) or “amiRNA HIF-1α” (A,B) induced by doxycycline using ELISA assay. The apoptotic cells were observed and calculated in T24/5637 cells treated with “amiRNA β-catenin” (C–F) or “amiRNA HIF-1α” (C–F) induced by doxycycline using Hoechst 33,258 staining assay. Data are shown as mean ± SD.