Figure 1
From: Cancer-associated fibroblasts in early-stage lung adenocarcinoma correlate with tumor aggressiveness
Histological subtype and cellular composition of indolent and aggressive LUADs. (A) Design of the experiment. Using chest CT-scan analysis, LUAD patients were stratified into 2 groups with predicted indolent and aggressive tumor behavior. (Illustrations are created with BioRender.com.) (B) Representative images of histological subtypes identified in the experimental cohort (H&E staining, indolent n = 8, aggressive n = 7). The icons demonstrate distribution of the patients from indolent (green) and aggressive (red) groups with respect to identified histological subtypes of the LUAD tumor tissue. (C) Representative images of CHP staining showing deposition of collagen in the tumor tissue isolated from patients with indolent and aggressive LUADs. Corresponding quantification of collagen positive area in the LUAD tissue isolated from the patients (violine plot shows median collagen positive area; indolent n = 9, aggressive n = 7; *—p < 0.05). (D) Expression of canonical marker genes associated with major cell populations in the tumor microenvironment across identified cell clusters (scRNAseq data). (E) UMAP representation of the stromal cell population (indolent n = 6, aggressive n = 7). Projection of identified clusters within stromal cell populations (left). Projection of samples isolated from LUAD patients within stromal cell populations (middle). Projection of samples isolated from indolent and aggressive LUADs within stromal cell subpopulation (right). (F) Proportion of stromal cell subpopulations in relation to patients. (G) Proportion of stromal cell subpopulations in indolent and aggressive LUADs.
