Figure 3

Gut-associated bacterial communities of tadpoles exposed to native and translocated waters sources over time: (a) phylogenetic α-diversity detected in each host species, Bufo spinosus (top) and Pelophylax perezi (bottom); and (b) β-diversity (using unweighted Unifrac distances) detected among tadpoles of each species. Yellow and green colour shades represent the water source (Gafanha and Lousada, respectively), ellipses denote the four experimental groups, and symbols indicate sampling events. P. perezi tadpoles were collected at Gafanha and B. spinosus tadpoles at Lousada, which correspond to their respective native source waters. Values of Linear Hypothesis test (LHT) and Power analysis for each GLMM are indicated within each α-diversity plot.