Figure 2
[Ca2]i is increased by cafedrine/theodrenaline, cafedrine alone, and theodrenaline alone. Dose–response relationships of (A) 20:1 cafedrine/theodrenaline, (C) cafedrine alone, and € theodrenaline are described by the Hill equation. (B) Cafedrine/theodrenaline (0.38 mM), (D) cafedrine alone (3.14 mM), and (F) theodrenaline alone (3.45 mM) induced a steep increase in [Ca2]i; however, [Ca2]i rapidly returned to its baseline value despite the continued presence of the applied substances. Application of potassium chloride (200 mM) confirmed the vitality of the included cells (not shown). FURA-2 340/380 ratio was normalized after a 100-s resting period, and each group consists of 30 cells from at least three different coverslips, ensuring independent measurements. Scale bar width represents 100 s. ⊥ SEM.
