Figure 5
Ca2+ release occurs independently from mitochondrial Ca2+ stores. When mitochondrial stores were depleted by 2,4-dinitrophenol (DNP, 25 µM), [Ca2+]i sharply increased following the application of (A, B) 20:1 cafedrine/theodrenaline, (C, D) cafedrine alone, and (E, F) theodrenaline alone. [Ca2+]i did not return to its baseline value within the observation period. The Ca2+-free control group was represented by the same 30 cells in each chart. FURA-2 340/380 ratio was normalized after a 100-s resting period, and each group consists of 30 cells from at least three different coverslips, ensuring independent measurements. Scale bar width represents 100 s. n = number of individual cells, ***p < 0.001, ns: not significant, Mann–Whitney U test, adjusted α-level = 0.017. ⊥ SEM, box and whisker plots indicate median, interquartile range (box), minimum and maximum (whiskers).
