Figure 7 | Scientific Reports

Figure 7

From: A fixed 20:1 combination of cafedrine/theodrenaline increases cytosolic Ca2+ concentration in human tracheal epithelial cells via ryanodine receptor-mediated Ca2+ release

Figure 7

Ca2+ release from intracellular stores occurs independently from IP3 receptor activation. When IP3 receptors were inhibited using 2-APB (40 µM), maximum [Ca2]i increased equally after the application of (A, B) 20:1 cafedrine/theodrenaline, (C, D) cafedrine alone, and (E, F) theodrenaline alone. The Ca2+-free control group was represented by the same 30 cells in each chart. FURA-2 340/380 ratio was normalized after a 100-s resting period, and each group consists of 30 cells from at least three different coverslips, ensuring independent measurements. Scale bar width represents 100 s. n = number of individual cells, ***p < 0.001, ns: not significant, Wilcoxon rank sum test was used to test 2-APB alone vs. drug preparation during inhibition, otherwise Mann–Whitney U test, adjusted α-level = 0.013. SEM, box and whisker plots indicate median, interquartile range (box), minimum and maximum (whiskers).

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